Detection of Y-chromosome microdeletions in infertile Libyan men using multiplex PCR in misurata
Teka, Ibrahim Ali
Alzalouk, Marwa mohammed
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Infertility is considered as one of the main health problems affecting about 10- 15% of couples seeking children. However, in 50% of these the male partner is responsible for infertility.Y chromosome classical microdeletions in the azoospermia factor (AZF) regions are known to be associated with spermatogenic failure. Spermatogenesis is an essential reproductive process that is regulated by many Y chromosome specific genes. The Y-chromosome is the smallest chromosome that consists of 2-3% of the haploid genome and may contain between 70 and 200 genes. This study was carrying out to understand prevalence and patterns microdeletions in azoospermia factor (AZF) region of Y chromosome in infertile Libyan men using PCR. In the current study, 50 patients from male infertility and 10 healthy controls. were studied in order to explore the Y chromosome disorders of male infertility in Misurata of Libya. AZF microdeletions and their associated phenotypes in infertile males have been extensively studied, have been identified by sequence-tagged site polymerase chain reaction (STS-PCR). The results of this study showed deletions in two cases ( 3.33%) as one of them had a deletion in AZFc and AZFd regions and the other had a deletion in AZFd region. our study showed that the prevalence of Y chromosome microdeletions is 3.33% in our population , also our study proves that molecular analysis is mandatory in any diagnostic workup of idiopathic infertile males. Moreover,. the importance of examining a molecular genetics approach including AZF deletions must be emphasized for those men who are considering intra cytoplasmic sperm injection ICSI, because this genetic defect is transmitted to their sons, affecting their fertility.