EVALUATION OF ANTI-INFLAMMATORY EFFECT OF IBUPROFEN USING WITEPOSOL H15 RECTAL SUPPOSITORIES VERSUS ORAL ROUTE

Abstract

Inflammation is the self-protective reaction of tissues towards infection, irritants, or foreign substances. Though it is a part of host defense mechanism, when it becomes severe, it turns out to be a hopeless condition which causes damage of tissues; hence control of inflammation becomes essential. This paper presents a study of anti-inflammatory effect of ibuprofen-witepsol H15 rectal suppositories versus oral route of administration using intra-peritoneal (IP) route as positive control. Suppositories were prepared using fusion method and tested for physico-chemical properties according to British Pharmacopeia (B.P) 2007 procedures. Screening for anti-inflammatory activity of ibuprofen was carried out against carrageenan-induced rat paw oedema in wistar strain albino rats (200 ± 20 g) of either sex. The formulated suppositories were found to satisfy B.P, requirements for weight variation, liq-uification time, disintegration time, hardness values and content uniformity. The entrapment efficiency (%) of ibu-profen was 99-100 % w/w for all the tested suppositories. The increase in paw oedema volume significantly (p< 0.0001) inhibited using ibuprofen-witepsol H15 rectal suppositories compared to control. The total area under the curve (AUC) for paw volumes time curve were reduced from 5.54 ml/h for control to 3.45, 4.38 and 4.78 ml/h using rectal suppositories, oral and IP routes. The anti-inflammatory inhibition (%) achieved after 4-5 hours was 40.58, 29.87 and 65.87, after rectal suppositories, oral and I.P routes of administration respectively. The total AUC (%) of inhibition were 33.81, 27.07 and 62.16 at 4 and 5 hours. using, rectal suppositories, oral and IP routes of administra-tion respectively. The results justified the use of suppositories in the treatment of inflammation when oral route is unreliable.