Development and validation of UV spectrophotometer method for the quick estimation of olive leaf extract (OLE)in pharmaceutical formulation
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Date
2015-12Author
Faisal, Mohammad Shah
Alhssony, Zainab Mohammed
Algneedy, Samia Mohammed
Elfytury, Khadija Abdullah
Naz, Zrien
El-Rutub, Mohammad
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Olive (Olea europaea), a rich source of natural antioxidant is native to the Mediterranean region. The main active constituents of olive include oleic acid, phenolic constituents like hydroxytyrosol and oleuropein and squalene are abundantly found in its leaf. Therefore, the aim of our research is to develop and validate simple, sensitive and specific spectrophotometric method for olive leaf extract (OLE). The developed method was validated with respect to linearity, accuracy, precision and specificity. The adequate drug solubility and maximum sensitivity were found in ethanol. The λmax or the absorption maxima of the drug was found to be 279 nm. The samples were prepared in ethanol and method obeyed Beers law in concentration ranges 5- 50 μg/ml. The content of OLE in herbal pharmaceutical formulation was determined. The results of analysis were validated statistically and confirmed the accuracy of the proposed method. Hence the proposed method can be used for the reliable quantification of olive leaf extract in herbal pharmaceutical formulation.