طيف β – lactamase ينتج Enterobacteriaceae المعزول من الدجاج في مصراتة

Abstract

Extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae in animals has a major concern due to the possible transmission between them and human that may cause a public health threat. Our study is conducted to estimate the prevalence of resistance pattern and characterization of ESBL producibility and genes in Enterobacteriaceae isolated from chicken feces from poultry farms in Misurata city. 135 fecal samples from 45 chicken farms were collected. Isolation and identification of different Enterobacteriaceae strains were performed to these isolates. In addition, Antibiotic Resistance Profile (ARP) against β-lactam antibiotics, ESBL phenotypic screening and PCR for ESBL genes; CTX, SHV and TEM, were done. The results showed that 88.8 of chicken isolates confirmed to be Enterobacteriaceae. 45% of chicken Enterobacteriaceae isolates were proved to be ESBL producing. The frequency of ESBL genes showed that multigene producers were 66.7% (36/54). CTX gene was detected in 47 (87%) isolates and TEM gene accounts for 39 (72.2 %) isolates, while the least proportion was for SHV 20 (37 %) isolates. From previous study we can conclude that the prevalence of ESBL Enterobacteriaceae and their genes in poultry farms of Misurata city would account for economic and public health threat in the society